Assays

Robust and reproducible protocols for HTS
Optimized for 48, 96 and 384 multiwell assays
Acute and chronic liver effects of any compounds
Primary or transformed human hepatocytes

Toxicity (IC50 determination)

Cell viability assays

Hepatocyte functionality

Drug metabolism

mRNA expression and activities

Apoptosis

Cell proliferation

EdU incorporation and mitotic index

Genotoxicity and mutagenesis

ΓH2AX quantification, comet assay, micronucleus assay

Liver pathologies

Steatosis
Quantification of lipids overload

Liver pathologies

Cholestasis
Drug-induced bile acids accumulation toxicity

Liver pathologies

Assay of collagen deposition